![]() Because gRNAs are 20 nucleotides long, the potential off-targets are limited to closely related sequences, hence off-site cleavage is relatively predictable and potentially avoidable. Several factors can impact how accurately the gRNA directs CRISPR effector protein cleavage. The first is the accuracy of the technique or the potential of damage to “off-targets”. There are two principal limitations of CRISPR. Successful treatment of sickle cell anemia. ![]() ![]()
0 Comments
Leave a Reply. |